techniques for ovarian tissue, whole ovary, oocyte and embryo cryopreservation
نویسندگان
چکیده
in recent years, preservation of fertility in women has been of great importance, especially in patients exposed to deleterious conditions on fertility. thus, cryopre-servation of human gametes, embryos and ovarian tissue has become an essential part of assisted reproduction. this approach limits the number of embryos transferred, while supernumerary oocytes and/ or embryos can be used in subsequent treatment cycles. furthermore, cryopreservation reduces the potential risk of hyperstimulation syndrome. cryopreservation is carried out by two techniques; the slow freezing method, and the more recent rapid procedure called vitrification technology. recently due the success and simplicity of vitrification, the balance between those two methods has been changed in advantage of vitrification. the use of slow freezing method has become controversial due to its difficulties, expense and respective low success rates in artificial reproduction. therefore, vitrification seems to win the battle and will be the cryopreservation method of the future.
منابع مشابه
Techniques for Ovarian Tissue, Whole Ovary, Oocyte and Embryo Cryopreservation
In recent years, preservation of fertility in women has been of great importance, especially in patients exposed to deleterious conditions on fertility. Thus, cryopreservation of human gametes, embryos and ovarian tissue has become an essential part of assisted reproduction. This approach limits the number of embryos transferred, while supernumerary oocytes and/ or embryos can be used in subseq...
متن کاملOvarian tissue and oocyte cryopreservation
There are relatively few effective clinical options for preserving female fertility, particularly following aggressive chemotherapy and/or radiotherapy treatment protocols. This document reviews the scientific background, current technology, clinical results and potential future applications of two methods for preserving female fertility—ovarian tissue cryopreservation and oocyte cryopreservati...
متن کاملHuman Oocyte And Embryo Cryopreservation
The technology so far applied clinically has been based directly on traditional human embryo cryopreservation protocols, and has produced relatively few offspring. Fortunately to date, no abnormalities have been reported from these pregnancies, regardless of the persistent concerns that freezing and thawing of mature oocytes may disrupt the meiotic spindle and thus increase the potential for an...
متن کاملFresh ovarian tissue and whole ovary transplantation.
A series of monozygotic (MZ) twin pairs discordant for premature ovarian failure presented an unusual opportunity to study ovarian transplantation. Ten MZ twin pairs requested ovarian transplantation and nine have undergone transplantation with cryopreservation of spare tissue. Eight had a fresh cortical tissue transplant, one of whom received a second frozen-thawed transplant after the first c...
متن کاملOvarian tissue viability following whole ovine ovary cryopreservation: assessing the effects of sphingosine-1-phosphate inclusion.
BACKGROUND Cryopreservation is hypothesized to result in apoptosis, contributing to stromal damage and follicle loss in ovarian tissue. This study investigated tissue viability following whole ovine ovary cryopreservation and examined the effects of the anti-apoptotic agent sphingosine-1-phosphate (S-1-P) on ovarian cryopreservation efficiency. METHODS Whole ovine ovaries were cryoperfused an...
متن کاملTowards whole sheep ovary cryopreservation q
Cryopreservation of ovarian tissue aims to assist young women who require treatments that may lead to sterility or infertility. Cryopreservation procedures should therefore be as simple and efficient as possible. This study investigates rapid cooling outcomes for whole sheep ovaries. Ovaries were perfused with VS4 via the ovarian artery, and cooled by quenching in liquid nitrogen in less than a...
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عنوان ژورنال:
journal of reproduction and infertilityجلد ۱۱، شماره ۱، صفحات ۳-۱۱
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